Oxygen
Saturation Analysis of Hemoglobin and Myoglobin
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Excel (xls) Spreadsheets
Analysis of Hb and Mb O
2
by Saturation and Hill Plots
Multifactor Regulation of Hb O
2
Saturation
Graphical Formats
Rectangular
Hyperbolic Plot
Hill Plot
Scatchard Plot
Y-axis
vs.
X-axis
Ya
vs.
pO
2
log (Ya/Yd)
vs.
log (pO
2
)
r/pO
2
vs.
r
where
r
=
v
*Ya
and
v
=
valence
MSOWC
1
Charts
Experimental
&
Theoretical O
2
Plots
Hemoglobin A vs Myoglobin O
2
Saturation Plot
Hemoglobin vs. Myoglobin - Hill Plot
O
2
Saturation and Hill Plots
for Mb & Hb
Hemoglobin vs. Myogbloin - Scatchard Plot
Hill Equation: Ya
=
pO
2
n
/ ( P
50%
n
+ pO
2
n
)
Hill Coefficient =
n
P
50%
= pO
2
at 50% saturation
1
MSOWC
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©
Duane W. Sears
Revised: July 25, 2001