| Human
Lysozyme Complex with Hydrolyzed
NAG6 (1lzs.pdb) NAG4 (in sites A-D) + NAG2 (in sites E-F) |
| Reference: Structural changes of active site cleft and different saccharide binding modes in human lysozyme co-crystallized with hexa-N-acetyl-chitohexaose at pH 4.0. Song, H., Inaka, K., Maenaka, K., Matsushima, M.: Structural changes of active site cleft and different saccharide binding modes in human lysozyme co-crystallized with hexa-N-acetyl-chitohexaose at pH 4.0. J Mol Biol 244 pp. 522 (1994) |
| Partial Abstract: "Human lysozyme was co-crystallized with hexa-N-acetyl-chitohexaose, (GlcNAc)6... (GlcNAc)6 had already been cleaved to (GlcNAc)4 and (GlcNAc)2.... (GlcNAc)4 was bound to the A, B, C, and D subsites, and binding sites of (GlcNAc)2 were close to the E and F subsites proposed on the basis of model building by Phillips and his colleagues. ...the three-dimensional structures remain in intermediate states between a transition state and an enzyme/product complex state." |
| Catalytic Residues | Hydrolyzed Substrate | Function |
| Asp53-COO- Glu35-COOH |
(GlcNAc)4+(GlcNAc)2 in sites A-D in sites E-F |
The hydrolysis of chitin. |